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HRID985933

反应详情

EQUATION

反应方程式

HRID 985933 的结构方程式

REACTANTS

反应物

2
结构：COC(=O)CC(=O)CCl
HCID36240

Methyl 4-chloroacetoacetate

C5H7ClO3

结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID439153

Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide

C21H29N7O14P2

PRODUCTS

生成物

1
结构：COC(=O)C[C@@H](O)CCl
HCID10920707

Methyl (3R)-4-chloro-3-hydroxybutanoate

C5H9ClO3

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O=P([O-])([O-])[O-].[Na+].[Na+].[Na+]
溶剂HCID24243

Trisodium phosphate

Na3O4P

PROCEDURE

实验过程

Methyl-4-chloroacetoacetate (VII) (100 mg) was incubated with 29 units of porcine heart (EC 1.1.1.35), B-hydroxyacyl CoA dehydrogenase (Sigma, H4626), and 1.36 g of NADH (Sigma, 90%) in 30 ml of 0.1 M sodium phosphate buffer, pH 6.5.