Pyruvic Acid → N-Acetyl-Neuraminic Acid

HRID 482209

Reaktionsgleichung

Reaktionsgleichung von HRID 482209
[C:1]([NH:4][C@@H:5]1[C@@H:11]([OH:12])[C@H:10]([OH:13])[C@@H:9]([CH2:14][OH:15])[O:8][CH:6]1[OH:7])(=[O:3])[CH3:2].[C:16]([OH:21])(=[O:20])[C:17]([CH3:19])=[O:18].P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.[Mg+2].[Cl-].[Cl-]>C(O)C(N)(CO)CO.Cl>[C:1]([NH:4][C@H:5]1[C@H:11]([C@@H:10]([C@@H:9]([CH2:14][OH:15])[OH:8])[OH:13])[O:12][C:17]([OH:18])([C:16](=[O:21])[OH:20])[CH2:19][C@@H:6]1[OH:7])(=[O:3])[CH3:2]

Komponenten

6

Edukt 4

HCID 1060Pyruvic AcidHCID 59575'-AtpHCID 439174N-Acetyl-D-GlucosamineHCID 5360315Magnesium Chloride

Produkt 1

HCID 439197N-Acetyl-Neuraminic Acid

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 93573Tromethamine hydrochlorideLösungsmittel

Versuchsvorschrift

A solution prepared by dissolving 22 g of N-acetylglucosamine and 11 g of pyruvic acid in 50 mM of Tris-HCl buffer (pH 7.5) containing 5 mM ATP and 5 mM MgCl2. To the solution was added 15 mg of protein having renin binding activities obtained in example 3 and 500 unit of N-acetylneuraminic acid lyase, and the total volume of the solution was adjusted to 0.5 liter. The mixture was reacted at 30° C. for 48 hours. After the reaction, 12.4 g of N-acetylneuraminic acid was produced in the reaction mixture. Reaction product was isolated by ion-exchange chromatography with Dowex 11 (DOW CHEMICAL CO.). After concentration, 10.3 g of N-acetylneuraminic acid was obtained by lyophilization.

Aufarbeitung

  1. customA solution prepared
  2. additionTo the solution was added 15 mg of protein

Quellen und Nachweise

ORD-Eintrag
ord-6bc8bff3d0344a56a3c6b0633c83c3aa
Quelldatensatz
uspto-grants-1998_08
Patentnummer
US05795767

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