Thiamine Hydrochloride → L-Glutamine

HRID 268589

Reaction equation

HRID 268589 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[Cl-].[NH4+:14].P([O-])([O-])(O)=O.[K+].[K+].P([O-])(O)(O)=O.[K+].[OH2:28].[OH2:29].O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].OC(CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=O.[CH3:57][C:58]1[N+:62](CC2C=NC(C)=NC=2N)=CS[C:59]=1[CH2:72][CH2:73][OH:74].Cl.[Cl-]>O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Fe+2].O.O.O.O.O.S([O-])([O-])(=O)=O.[Mn+2].O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Zn+2]>[NH2:62][C@H:58]([C:57]([OH:29])=[O:28])[CH2:59][CH2:72][C:73](=[O:74])[NH2:14]

Participants

11

Reactant 7

HCID 6202Thiamine HydrochlorideHCID 24450Potassium Phosphate, DibasicHCID 24843Magnesium Sulfate HeptahydrateHCID 25517Ammonium ChlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphate

Product 1

HCID 5961L-Glutamine

Reagents, catalysts & solvents 3

HCID 62640Zinc Sulfate HeptahydrateCatalystHCID 62662Ferrous sulfate heptahydrateCatalystHCID 22138077Manganese II sulfate pentahydrateCatalyst

Procedure

Each of the strain ASL-7 and its parent strain ATCC14752 was cultured at 28° C. for 24 hours on the BYG agar medium. One loopful of the cultured cells were inoculated into a 300 ml capacity conical flask charged with 20 ml of a production medium (a medium prepared by dissolving 150 g of glucose, 50 g of ammonium chloride, 0.7 g of dipotassium hydrogenphosphate, 0.7 g of potassium dihydrogenphosphate, 0.5 g of magnesium sulfate heptahydrate, 20 mg of iron sulfate heptahydrate, 20 mg of manganese sulfate pentahydrate, 10 mg of zinc sulfate heptahydrate, 6 μg of biotin, 1 mg of thiamine hydrochloride and 5 g of meat extract in 1 liter of water, adjusting its pH to 7.2 and then adding 50 g of calcium carbonate thereto) and cultured at 28° C. for 96 hours.

Workup

  1. additioncharged with 20 ml of a production medium (a medium
  2. customprepared
  3. extractionextract in 1 liter of water
  4. additionadding 50 g of calcium carbonate
  5. wait) and cultured at 28° C. for 96 hours

Sources & evidence

ORD record
ord-a35d2c485bfd472ba49de6dc317d6d95
Source dataset
uspto-grants-2008_11
Patent number
US07445915B2

Linked notes

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