Phosphoenolpyruvate → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 405668

Reaction equation

HRID 405668 reaction equation
[Mg+2].[Cl-].[Cl-].[Cl-].[K+].C=C(OP(O)(O)=O)C(O)=O.P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.C([O-])(=O)C(C)=O.[O:53]=[C:54]1[O:60][C@H:59]([C@H:61]([CH2:63][OH:64])[OH:62])[C:57]([OH:58])=[C:55]1[OH:56]>O1CCN(CCS(O)(=O)=O)CC1>[O:53]=[CH:54][C@@H:55]([C@H:57]([C@@H:59]([C@@H:61]([CH2:63][OH:64])[OH:62])[OH:60])[OH:58])[OH:56]

Participants

8

Reactant 6

HCID 1005PhosphoenolpyruvateHCID 4873Potassium ChlorideHCID 59575'-AtpHCID 107735PyruvateHCID 5360315Magnesium ChlorideHCID 54670067L-Ascorbic Acid

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Reagents, catalysts & solvents 1

HCID 781654-Morpholineethanesulfonic acidSolvent

Procedure

In 10.0 mM 2-morpholinoethanesulfonic acid (MES) buffer, 17.6 mM MgCl2, 17.6 mM KCl, 175.7 mM phosphoenolpyruvic acid (PEP), 17.6 mM ATP, 123 U/mL pyruvate kinase (PK), 97 U/mL hexokinase, and 20 U/mL ascorbic acid oxidase were dissolved in this composition, and the pH was adjusted to 7.0 to obtain a glucose converting reagent.

Sources & evidence

ORD record
ord-e8dc61f2a583462c83b2582574c4ed4b
Source dataset
uspto-grants-2014_06
Patent number
US08753832B2

Linked notes

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