Benzyl Alcohol → (1S,2R,3S,4R)-3-benzyloxycarbonylamino-bicyclo[2.2.1]hept-5-ene-2-carboxylic acid methyl ester

温度

0 °C

(1R,2S,3R,4S)-3-(Methoxycarbonyl)bicyclo[2.2.1]hept-5-ene-2-carboxylic acid (4.61 g, 23.5 mmol) was dissolved in anhydrous tetrahydrofuran (40 mL). The flask was degassed and backfilled with nitrogen and the mixture was cooled to 0° C. Triethylamine (9.9 mL, 70.5 mmol) was added followed by the dropwise addition of ethyl chloroformate (4.48 mL, 47 mmol) with vigorous stirring. Immediate precipitation was observed. Additional tetrahydrofuran (60 mL) was added. The mixture was stirred at 0° C. for 1 h. Sodium azide (4.58 g, 70.5 mmol) was dissolved in water (30 mL) and added to the reaction mixture at 0° C. The mixture was stirred at 0° C. for 5 min. The ice bath was removed. The mixture was warmed to 25° C. and was stirred for 2 h. The mixture was poured into water (300 mL) and the product extracted into ethyl acetate (300 mL). The organic layer was further washed with half-saturated aqueous sodium bicarbonate solution (2×100 mL), saturated aqueous brine solution (100 mL), dried over magnesium sulfate, filtered, and concentrated in vacuo to afford a clear oil. The oil was dissolved in anhydrous benzene (50 mL) and refluxed while stirring under nitrogen for 2 h. Upon cooling to 25° C. the solution was concentrated in vacuo to afford a slightly yellow oil. The oil was dissolved in dichloromethane (30 mL) and benzyl alcohol (2.68 mL, 25.9 mmol) was added followed by triethylamine (6.61 mL, 47 mmol). The mixture was refluxed under nitrogen for 16 h. Upon cooling to 25° C. the solution was concentrated in vacuo to afford a golden oil. Purification by flash column chromatography (Merck silica gel 60, 40-63 μm, 15% ethyl acetate in hexanes) afforded the desired product, methyl (1S,2R,3S,4R)-3-{[(benzyloxy)carbonyl]amino}bicyclo[2.2.1]hept-5-ene-2-carboxylate (5.51 g, 18.31 mmol, 78%), as a clear oil. 1H NMR (400 MHz, CDCl3) δ: 1.38 (1H, d, J=9.1 Hz), 1.50 (1H, d, J=9.4 Hz), 3.10 (2H, s), 3.21 (1H, dd, J1=9.2 Hz, J2=2.3 Hz), 3.53 (3H, s), 4.62 (1H, dt, J1=9.4 Hz, J2=2.9 Hz), 5.07 (2H, q, J=13.0 Hz), 5.29 (1H, d, J=8.6 Hz), 6.15-6.17 (1H, m), 6.37-6.38 (1H, m), 7.29-7.35 (5H, m). LC-MS (ESI) calcd for C17H19NO4 301.13. found 258.1 (100%), 302.2 [M+H+] (70%), 603.5 [2M+H+] (20%).

1. customThe flask was degassed
2. customImmediate precipitation
3. additionAdditional tetrahydrofuran (60 mL) was added
4. additionadded to the reaction mixture at 0° C
5. stirringThe mixture was stirred at 0° C. for 5 min
6. customThe ice bath was removed
7. temperatureThe mixture was warmed to 25° C.
8. stirringwas stirred for 2 h
9. additionThe mixture was poured into water (300 mL)
10. extractionthe product extracted into ethyl acetate (300 mL)
11. washThe organic layer was further washed with half-saturated aqueous sodium bicarbonate solution (2×100 mL), saturated aqueous brine solution (100 mL)
12. dry with materialdried over magnesium sulfate
13. filtrationfiltered
14. concentrationconcentrated in vacuo
15. customto afford a clear oil
16. temperaturerefluxed
17. stirringwhile stirring under nitrogen for 2 h
18. temperatureUpon cooling to 25° C. the solution
19. concentrationwas concentrated in vacuo
20. customto afford a slightly yellow oil
21. temperatureThe mixture was refluxed under nitrogen for 16 h
22. temperatureUpon cooling to 25° C. the solution
23. concentrationwas concentrated in vacuo
24. customto afford a golden oil
25. customPurification by flash column chromatography (Merck silica gel 60, 40-63 μm, 15% ethyl acetate in hexanes)

ORDレコード

ord-10f3186b5c0d480f8b4b4caadeb7bf3c

出典データセット

uspto-grants-2013_10

DOI

10.6084/m9.figshare.5104873.v1

特許番号

US08546602B2