Adenine → Camp

HRID 1200095

反応式

HRID 1200095 の反応式
CC(OC1C2(C)CCC3C4C=CC(OC(C)=O)=CC=4CCC3C2CC1)=O.[Na+].[Cl-].[Cl-].[K+].O=C[C@@H:33]([C@H:35]([C@@H:37]([C@@H:39]([CH2:41][OH:42])[OH:40])[OH:38])[OH:36])O.[P:43]([O-:47])([O-])(O)=[O:44].[Na+].[Na+].C1C=CC2S(=O)(=O)OC(C3C=CC(O)=CC=3)(C3C=CC(O)=CC=3)C=2C=1.[O-]S([O-])(=O)=O.[Mg+2].[Cl-].[Cl-].[Ca+2].CC(CN1C(=O)N(C)C(=O)C2N=CNC1=2)C.[N:100]1[C:108]([NH2:109])=[C:107]2[C:103]([N:104]=[CH:105][NH:106]2)=[N:102][CH:101]=1>C(=O)=O.C(O)(=O)C>[CH:101]1[N:100]=[C:108]([NH2:109])[C:107]2[N:106]=[CH:105][N:104]([C@@H:33]3[O:40][C@@H:39]4[CH2:41][O:42][P:43]([OH:47])([O:38][C@H:37]4[C@H:35]3[OH:36])=[O:44])[C:103]=2[N:102]=1

参加物

13

反応物 10

HCID 190AdenineHCID 3264beta-Estradiol diacetateHCID 4766Phenol RedHCID 4873Potassium ChlorideHCID 5234Sodium ChlorideHCID 24083Sulfuric acid magnesium salt (1:1)HCID 24203Sodium Phosphate, DibasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 5284359Calcium ChlorideHCID 178448723HCID 178448723、化合物

生成物 1

HCID 6076Camp

試薬・触媒・溶媒 2

HCID 176Acetic Acid溶媒HCID 280Carbon Dioxide溶媒

実験手順

Uterus homogenate is prepared as described in Example 5 from rats treated with beta-estradiol diacetate. The buffer used for the homogenization is composed of 0.14 mol/l NaCl, 5 mmol/l KCl, 10 mmol/l glucose, 10 mmol/l acetic acid, 2 mmol/l disodium hydrogen phosphate, 20 mmol/l tris-HCl, 0.001% phenol red, 1.2 mol/l MgSO4, 0.8 mmol/l CaCl2 and 0.01 mmol/l isobutylmethylxanthine. Before use, this buffer is brought to pH 7.4 by passing in CO2 gas. 10 ml of the uterus homogenate are now incubated with 0.3 mmol/l tritiated adenine at 37° C. for 40 minutes. The tissue is now filtered, again taken up in the above buffer, and subsequently divided up in such a way that each portion is suspended in 10 ml of the said buffer. After a preincubation period of 5 minutes, the active substances specified in Tab. 2 are added, and the mixture is incubated for a further 100 minutes. It is then filtered again, the tissue is deep-frozen in liquid nitrogen and crushed, and the resulting powder is homogenized in 1 ml of 6% strength trichloroacetic acid. Cyclic AMP is subsequently separated out and quantified by column chromatography as in Analytical Biochemistry 58, (1974) 541-548 (in particular Method C, page 543). Tab. 2 shows the results:

後処理

  1. customUterus homogenate is prepared
  2. filtrationThe tissue is now filtered
  3. customAfter a preincubation period of 5 minutes
  4. addition2 are added
  5. filtrationIt is then filtered again
  6. temperaturethe tissue is deep-frozen in liquid nitrogen
  7. customcrushed
  8. customCyclic AMP is subsequently separated out
  9. customresults

出典と根拠

ORDレコード
ord-ef4b491a428d434b83951c8ba5165ea2
出典データセット
uspto-grants-1990_02
特許番号
US04904648

関連ノート

読み込み中…