反応式
C[C@H]1O[C@@H]([O:8][C@:9]23[C:24](=[CH2:25])[CH2:23][C@:21]4([CH2:22]2)[C@H:12]([C@@:13]2([CH3:41])[C@H:18]([CH2:19][CH2:20]4)[C@@:17]([C:27]([O:29][C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)=[O:28])([CH3:26])[CH2:16][CH2:15][CH2:14]2)[CH2:11][CH2:10]3)[C@@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@@H](O)[C@@H]1O.[K].P([O-])([O-])([O-])=O.SC[C@H]([C@@H](CS)O)O.O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O>OP([O-])(O)=O.[K+].CC#N.C(Cl)(Cl)Cl.CO.O>[CH3:41][C@:13]12[C@@H:12]3[CH2:11][CH2:10][C@@:9]4([OH:8])[C:24]([CH2:23][C@@:21]3([CH2:22]4)[CH2:20][CH2:19][C@@H:18]1[C@@:17]([C:27]([OH:29])=[O:28])([CH3:26])[CH2:16][CH2:15][CH2:14]2)=[CH2:25]参加物
反応物 5
HCID 1061Phosphate
HCID 79025Alpha-D-Glucose
HCID 446094D-1,4-Dithiothreitol
HCID 5462222Potassium
HCID 44564216[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (1R,4S,5R,9S,10R,13S)-13-[(2S,3S,4S,5S,6R)-3-[(2S,3R,4S,5R,6R)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-4,5-dihydroxy-6-methyloxan-2-yl]oxy-5,9-dimethyl-14-methylidenetetracyclo[11.2.1.01,10.04,9]hexadecane-5-carboxylate2 回生成物 1
試薬・触媒・溶媒 3
実験手順
Rebaudioside C, 3-sulfopropyl ester, potassium salt prepared as in Part B. was incubated anaerobically for three days at 37° C. with 5% fresh rat cecal contents at concentrations of 0.25, 0.5, and 1.0 mg/ml, in sterile Krebs-Ringer 0.25 M phosphate buffer (pH 7.4) containing 0.25 mg/ml dithiothreitol and 0.25 mg/ml α-D-glucose. TLC [silica gel F-254; CHCl3 :CH3OH:H2O (15:10:2)] and HPLC [30 cm C-18 on μ-Bondapak; 15 min linear gradient of 10-40% CH3CN in 0.005 M KH2PO4 (pH 3.45); 200 nm] analysis showed all the rebaudioside C, 4-sulfopropyl ester (Rf =0.42; tR =14.0 min) to have been consumed within 24 h to yield apparently only the sulfopropyl ester of steviol, (Rf =0.63; tR =16.3 min). No steviol (Rf =0.95; tR =31.3 min) was detected. After 3 days the bacterial cells were sedimented by centrifugation. The sediment was extracted (THF), as was the lyophilized supernatant. HPLC analysis for steviol of the two THF extracts showed none to be detectable. With a detection limit of 0.05 μg, as little as 0.03 and 0.13 percent degradation to steviol could have been detected for the sediment and supernatant fractions, respectively.
後処理
- customto have been consumed within 24 h
出典と根拠
- ORDレコード
- ord-e88ee83601aa49e4a59a7ec3e76ed946
- 出典データセット
- uspto-grants-1982_10
- 特許番号
- US04353889
関連ノート
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