[N+:1]([C:4]1[CH:13]=[CH:12][C:7]2[S:8][CH2:9][CH2:10][NH:11][C:6]=2[CH:5]=1)([O-:3])=[O:2].Cl[CH2:15][C:16](Cl)=[O:17].Cl.[NH2:20][CH2:21][CH2:22][OH:23]>O1CCCC1.O.C(=O)([O-])[O-].[Na+].[Na+]>[OH:17][CH2:16][CH2:15][NH:20][CH2:21][C:22]([N:11]1[CH2:10][CH2:9][S:8][C:7]2[CH:12]=[CH:13][C:4]([N+:1]([O-:3])=[O:2])=[CH:5][C:6]1=2)=[O:23]To a stirred solution of 6-nitro-3,4-dihydro-2H-benzo[b][1,4]thiazine (500 mg, 2.55 mmol) in tetrahydrofuran (10 mL) was added 2-chloroacetyl chloride (302 mg, 2.68 mmol). The resulting mixture was stirred at 60° C. for 5 minutes. The reaction first turns cloudy then clarifies. At this time, the reaction was cooled to 0° C., and hydrogen chloride (4 M in dioxane; 5.10 mL, 20.38 mmol) and 2-aminoethanol (1.868 g, 30.6 mmol) were added to the reaction simultaneously in a dropwise fashion. The resulting mixture was warmed to room temperature, and water (5 mL) was added to aid dissolution. The mixture was then stirred at 60° C. for 3 hours. The mixture was then diluted with water and saturated sodium carbonate and then extracted with dichloromethane (5×). The combined organic layers were dried, filtered, concentrated, and then chromatographed in 1:4:5 (2M NH3 in methanol):ethyl acetate:dichloromethane giving the desired product (473 mg, 62.4%) as a pale yellow solid. 1H NMR (DMSO-d6) δ 8.36 (d, J=2.1 Hz, 1H), 7.93 (dd, J=8.7, 2.1 Hz, 1H), 7.50 (d, J=9.0 Hz, 1H), 4.50 (t, J=5.1 Hz, 1H), 3.96-3.90 (m, 2H), 3.56 (s, 2H), 3.48-3.40 (m, 2H), 3.32-3.26 (m, 2H), 2.58 (t, J=5.0 Hz, 2H), 2.18-2.08 (brs, 1H); ESI-MS (m/z, %): 298 (MH+, 100).
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