Sulfuric Acid → N-Methylphenylalanine

HRID 271040

반응식

HRID 271040 반응식
[C:1]1([CH2:7][C:8](=O)[C:9]([O-:11])=[O:10])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[Na+].[CH:14]1[N:15]=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3OP(O)(O)=O)C=2N=1.O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.CN.S(=O)(=O)(O)O.[OH-].[Na+]>>[CH3:14][NH:15][C@H:8]([C:9]([OH:11])=[O:10])[CH2:7][C:1]1[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1

참가 물질

7

반응물 6

HCID 1118Sulfuric AcidHCID 5884NADPHHCID 6329MethylamineHCID 14798Sodium HydroxideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2회HCID 23667645Sodium phenylpyruvate2회

생성물 1

HCID 6951135N-Methylphenylalanine

실험 절차

In a 50-mL beaker, 5.88 ml of 40 mM Tris-hydrochloride buffer (pH 8.0) containing 60 mg of sodium phenylpyruvate, 2.3 mg of NADPH, 35 U of glucose dehydrogenase, and 1 g of glucose was placed, to which 5.88 mL of 240 mM methylamine adjusted to pH 8.0 with sulfuric acid and 3.25 mL of the above cell-free extract were added, and the mixture was allowed to react at 30° C. The reaction was conducted while stirring and adjusting pH to 8.0 with 1 N aqueous sodium hydroxide solution. A portion of the reaction solution was analyzed by HPLC at regular intervals. When the substrate sodium phenylpyruvate was depleted, an additional 30 mg was added and the reaction was continued. This process was repeated 8 times during the reaction of 24 hours. Upon completion of the reaction, the amount of N-methylphenylalanine produced was 146 mg.

후처리

  1. extractioncell-free extract
  2. additionwere added
  3. customto react at 30° C
  4. additionan additional 30 mg was added
  5. customduring the reaction of 24 hours
  6. customUpon completion of the reaction

출처 및 근거

ORD 기록
ord-495ba43b2388481faeacbd3b18f0cc59
출처 데이터셋
uspto-grants-2008_11
특허 번호
US07452704B2

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