反应详情

To a solution of 130 mg (0.36 mmol) of tert-butyl (1S)-1-{(1S)-1-hydroxy-2-[(4-morpholinylcarbonyl)amino]ethyl}pentylcarbamate & tert-butyl (1S)-1-{(1R)-1-hydroxy-2-[(4-morpholinylcarbonyl)amino]ethyl}pentylcarbamate in 1 mL of dioxane was added 5 mL of 4M hydrochloric acid in dioxane. The reaction mixture was stirred for 2 h and concentrated under reduced pressure. Half of the product was carried forward. This material was slurried in N,N-dimethylformamide before 0.094 mL (0.54 mmol) of N,N-diisopropylethylamine was added, followed by 86 mg (0.18 mmol) of (1S)-2,2-dimethyl-1-({3-[4-(trifluoromethyl)phenyl]-1H-pyrazol-1-yl}methyl)propyl 4-nitrophenyl carbonate. The reaction mixture was stirred at room temperature for 18 h, and then diluted with ethyl acetate before being washed with 1M sodium hydroxide and brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel chromatography eluting with ethyl acetate to afford 60 mg (55%) of (1S)-2,2-dimethyl-1-({3-[4-(trifluoromethyl)phenyl]-1H-pyrazol-1-yl}methyl)propyl (1S)-1-{(1S)-1-hydroxy-2-[(4-morpholinylcarbonyl)amino]ethyl}pentylcarbamate & (1S)-2,2-dimethyl-1-({3-[4-(trifluoromethyl)phenyl]-1H-pyrazol-1-yl}methyl)propyl (1S)-1-{(1R)-1-hydroxy-2-[(4-morpholinylcarbonyl)amino]ethyl}pentylcarbamate. 1H-NMR (300 MHz, DMSO-d6): δ 7.99 (m, 2H), 7.74 (d, J=8 Hz, 2H), 6.77 (s, 1H), 6.58 (d, J=9 Hz, 1H), 6.33 (m, 1H), 4.95–4.75 (m, 2H), 4.48 (d, J=13 Hz, 1H), 4.19 (t, J=12 Hz, 1H), 3.45–3.10 (m, 13H), 1.25 (m, 1H), 0.99 (m, 14H), 0.67 (m, 3H); ES-LCMS m/z 620 (M+Na).

1. concentrationconcentrated under reduced pressure
2. additionwas added
3. stirringThe reaction mixture was stirred at room temperature for 18 h
4. washbefore being washed with 1M sodium hydroxide and brine
5. dry with materialdried over anhydrous magnesium sulfate
6. concentrationconcentrated under reduced pressure
7. customThe residue was purified by silica gel chromatography
8. washeluting with ethyl acetate