反应详情

温度

4 °C

A mixture of p-toluenesulfonic acid salt of Phe-Gly-OBzl (3.06 g), Boc-Gly-OH (1.10 g), N-hydroxysuccinimide (941 mg), N-methylmorpholine (0.725 ml), and N,N-dimethylformamide (40 ml) was cooled to 4° C., and added with N,N′-dicyclohexylcarboduimide (1.56 g). The mixture was allowed to react overnight at room temperature with stirring, and then evaporated to dryness under reduced pressure. The residue was purified by silica gel column chromatography (eluent: dichloromethane:methanol=98:2 solution) to give Boc-Gly-Phe-Gly-OBzl (1.93 g).

1. additionadded with N,N′-dicyclohexylcarboduimide (1.56 g)
2. customto react overnight at room temperature
3. customevaporated to dryness under reduced pressure
4. customThe residue was purified by silica gel column chromatography (eluent: dichloromethane:methanol=98:2 solution)