Thiamine Hydrochloride → L-Lysine

HRID 1728304Notes

Reaction equation

HRID 1728304 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[K].O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].S([O-])([O-])(=O)=O.[NH4+:32].[NH4+:33].[OH:34][C:35]([CH2:37][CH2:38][CH2:39][CH2:40][C@H:41]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:36].CC1[N+](CC2C(N)=NC(C)=NC=2)=CSC=1CCO.Cl.[Cl-]>[Fe].[Mn]>[NH2:32][C@H:37]([C:35]([OH:34])=[O:36])[CH2:38][CH2:39][CH2:40][CH2:41][NH2:33]

Participants

9

Reactant 6

HCID 6202Thiamine HydrochlorideHCID 24843Magnesium Sulfate HeptahydrateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 5462222PotassiumHCID 6097028Ammonium Sulfate

Product 1

HCID 5962L-Lysine

Reagents, catalysts & solvents 2

HCID 23925IronCatalystHCID 23930ManganeseCatalyst

Procedure

An aqueous solution medium containing 15% of glucose, 0.1% of potassium primary phosphate (KH2PO4), 0.04% of magnesium sulfate heptahydrate, 2% of ammonium sulfate, 100 μg/l of biotin, 200 μg/l of vitamin B1 hydrochloride, 2 ppm each of iron ions and manganese ions and 1% of "AJI-EKI" pH 7.0, was separately charged in a small glass jar fermenters in an amount of 300 ml each. After sterilization, Lys-producing Brevibacterium flavum (ATCC 21127), which had been previously grown in a bouillon slant at 30° C. for 24 hours, was inoculated on the medium. Then incubation was initiated at 31° C. About 10 hours after, penicillin was supplemented in a concentration of 6 U/ml and the incubation was continued for 18 hours. At the end of the fermentation, 3.75 g/dl of L-Lysine and 2.05 g/dl of L-glutamic acid were produced and accumulated in the medium.

Workup

  1. additionwas separately charged in a small glass jar fermenters in an amount of 300 ml each

Sources & evidence

ORD record
ord-38287d6286e14c4b805253b7d3280055
Source dataset
uspto-grants-1994_07
Patent number
US05326693

Reaction notes

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