反应详情

To a solution of 2.75 g 1-phenyl-3-carboxy-5-pyrazolone and 5.00 g 4-((S)-2-amino-4-tert-butoxycarbonyl-butyryl)-piperazine-1-carboxylic acid butyl ester in 15 ml DMF 2.06 g HOBt, 4.5 ml DIPEA and 2.58 g EDC were added and the reaction mixture was stirred for 12 h at RT. Then the reaction mixture was diluted with ethyl acetate and subsequently extracted with aqueous LiCl (4% w/w), 0.1 M HCl and aqueous NaHCO3. The organic layer was dried over MgSO4 and the solvent was removed under reduced pressure. The crude product thus obtained was used in the subsequent reaction.

1. extractionsubsequently extracted with aqueous LiCl (4% w/w), 0.1 M HCl and aqueous NaHCO3
2. dry with materialThe organic layer was dried over MgSO4
3. customthe solvent was removed under reduced pressure