反应详情

温度

23 °C

A suspension of NaH (60% dispersion in mineral oil (17 mg, 423 μmol)) in dioxane (2.5 mL) was treated with a solution of (E)-benzaldehyde oxime (51 mg, 423 μmol) in DMF (1.5 mL). The reaction was stirred at 23° C. under nitrogen. After 30 minutes, the reaction was cooled to 0° C. and then a solution of 1-tert-butyl ethyl 4-(4-chloro-2-(3-ethoxy-3-oxoprop-1-ynyl)phenyl)piperidine-1,4-dicarboxylate (157 mg, 338 μmol) in DMF (3.5 mL) was added in a dropwise fashion over 10 minutes. After 1 hour, the reaction mixture was diluted with EtOAc (100 mL) and washed with saturated NaHCO3 solution (75 mL), water (75 mL) and brine (75 mL), dried over MgSO4, concentrated in vacuo, and purified by silica gel chromatography (eluant: 1-8% MeOH/DCM), affording the cyclized product. The cyclized product was mixed with glycine methyl ester hydrochloride (28 mg, 220 μmol) and DIPEA (48 μL, 275 μmol) in 1,4-dioxane (4 mL) and heated to 95° C. in a sealed vessel. After 18 hours, the reaction was cooled to 23° C., diluted with EtOAc (75 mL) and washed with saturated NaHCO3 solution (50 mL) and brine (50 mL), dried over MgSO4, concentrated in vacuo, and purified by silica gel chromatography (eluant: 28-100% EtOAc/hexane) affording the title compound in 40 mg. MS m/e=501.2 (M+Na)+.

1. temperaturethe reaction was cooled to 0° C.
2. waitAfter 1 hour
3. washwashed with saturated NaHCO3 solution (75 mL), water (75 mL) and brine (75 mL)
4. dry with materialdried over MgSO4
5. concentrationconcentrated in vacuo
6. custompurified by silica gel chromatography (eluant: 1-8% MeOH/DCM)
7. customaffording the cyclized product
8. temperatureheated to 95° C. in a sealed vessel
9. waitAfter 18 hours
10. temperaturethe reaction was cooled to 23° C.
11. washwashed with saturated NaHCO3 solution (50 mL) and brine (50 mL)
12. dry with materialdried over MgSO4
13. concentrationconcentrated in vacuo
14. custompurified by silica gel chromatography (eluant: 28-100% EtOAc/hexane)