Ethylenediaminetetraacetic Acid → HCID 22846432, compound

HRID 2254522Notes

Reaction equation

HRID 2254522 reaction equation
ON1C(=O)CCC1=O.O[C:10]([CH2:12][CH2:13][CH2:14][CH2:15][C@H:16]1[C@@H:24]2[C@@H:19]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:17]1)=[O:11].[SH:25][CH2:26][CH2:27][NH2:28].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.C(O)C(N)(CO)CO.Cl>CN(C)C=O.P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[C:10]([NH:28][CH2:27][CH2:26][SH:25])(=[O:11])[CH2:12][CH2:13][CH2:14][CH2:15][C@H:16]1[C@@H:24]2[C@@H:19]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:17]1

Participants

7

Reactant 4

HCID 6049Ethylenediaminetetraacetic AcidHCID 6058CysteamineHCID 93573Tromethamine hydrochlorideHCID 86743017n-Hydroxysuccinimide biotin

Product 1

HCID 22846432HCID 22846432, compound

Reagents, catalysts & solvents 2

HCID 6228DimethylformamideSolventHCID 24243Trisodium PhosphateSolvent

Procedure

An aliquot (0.1 ml) of 44 mM biotin-N-hydroxysuccinimide (Zymed Laboratories, Inc., San Francisco, Calif.) in N,N-dimethylformamide was incubated with 1.0 ml of 4.4 mM 2-mercaptoethylamine in 0.1M sodium phosphate buffer, pH 7.0, containing 5 mM EDTA, at 30° C. for 30 minutes, and 0.1 ml of 1M Tris-HCl buffer, pH 7.0, was added.

Sources & evidence

ORD record
ord-aed93820891941cbb1716788f3ab9c12
Source dataset
uspto-grants-1993_08
Patent number
US05236849

Reaction notes

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