反应详情

An aliquot (0.1 ml) of 44 mM biotin-N-hydroxysuccinimide (Zymed Laboratories, Inc., San Francisco, Calif.) in N,N-dimethylformamide was incubated with 1.0 ml of 4.4 mM 2-mercaptoethylamine in 0.1M sodium phosphate buffer, pH 7.0, containing 5 mM EDTA, at 30° C. for 30 minutes, and 0.1 ml of 1M Tris-HCl buffer, pH 7.0, was added.