反应详情

To 0.16 mole of t-butoxycarbonylalanylproline in 80 ml of dry tetrahydrofuran which has been cooled to -20° to -30° is added 2 ml of N-methylmorpholine and then 2.1 ml of isobutylchloroformate. After stirring for 10 minutes, 0.16 mole of 2-azaalanine ethyl ester and 60 ml of chloroform are added. The reaction mixture is allowed to warm to room temperature overnight, concentrated in vacuo, taken up in ethyl acetate and the insoluble material removed by filtration. Concentration of the filtrate in vacuo gives t-butoxycarbonylalanylprolyl-2-azaalanine ethyl ester.

1. concentrationconcentrated in vacuo
2. customthe insoluble material removed by filtration