huagongshe.com
HRID438035

反应详情

EQUATION

反应方程式

HRID 438035 的结构方程式

REACTANTS

反应物

2
结构：CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
HCID44259

Staurosporine

C28H26N4O3

结构：N#Cc1ccc(C=O)cc1Br
HCID53425519

2-Bromo-4-formylbenzonitrile

C8H4BrNO

PRODUCTS

生成物

4
结构：c1ccc(-c2ccccc2)cc1
HCID7095

Biphenyl

C12H10

结构：CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
HCID44259

Staurosporine

C28H26N4O3

结构：N#Cc1ccc(CO)cc1Br
HCID288434

2-Bromo-4-(hydroxymethyl)benzonitrile

C8H6BrNO

结构：CO[C@@H]1[C@H](N(C)Cc2ccc(C#N)c(Br)c2)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
HCID178496679

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

4
结构：CC(=O)O
试剂HCID176

Acetic Acid

C2H4O2

结构：CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].[Ti+4]
试剂HCID11026

2-Propanol, titanium(4+) salt

C12H28O4Ti

结构：CC(=O)O[BH-](OC(C)=O)OC(C)=O.[Na+]
试剂HCID5049666

Sodium triacetoxyborohydride

C6H10BNaO6

结构：CC(=O)N(C)C
溶剂HCID31374

Dimethylacetamide

C4H9NO

7 次

CONDITIONS

反应条件

温度
22 °C

WORKUP

后处理

  1. additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
  2. additionThe reactions were diluted with 3x1 uL DMSO (containing PhPh as an internal standard) using the Mosquito(R). The reactions were mixed after each dispense operation.
  3. aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
  4. additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.