反应详情

Weighed into a 1.5 ml Eppendorf tube were 1.8 mg (3 μmol) of benzylguanine-N-Fmoc-piperidine which was dissolved in 190 μl of anhydrous dimethylformamide. 10 μl of piperidine were added and the reaction mixture was left stirring for one hour. The reaction was monitored by HPLC on a Merck Lichrospher RP °18, 5 μm, 125×4.6 column with a gradient of acetonitrile in water containing 0.2% of trifluoroacetic acid.

1. waitthe reaction mixture was left