反应详情

(2S,3S,5S)-2-Amino-3-hydroxy-5-t-butyloxycarbonylamino-1,6-diphenylhexane succinate salt (30 g, 63 mmol; U.S. Pat. No. 5,654,466), ((5-thiazolyl)methyl)-(4-nitrophenyl)carbonate hydrochloride (22.2 g; U.S. Pat. No. 5,597,926) and sodium bicarbonate (16.2 g) were mixed with 300 mL of water and 300 mL of ethyl acetate and the mixture was stirred at room temperature for about 30 minutes. The organic layer was then separated and heated at about 60° C. for 12 hours, and then stirred at 20–25° C. for 6 hours. 3 mL of ammonium hydroxide (29% ammonia in water) was added and the mixture stirred for 1.5 hours. The resulting mixture was washed with 4×200 mL of 10% aqueous potassium carbonate and the organic layer was separated and evaporated under vacuum to provide an oil. The oil was suspended in about 250 mL of heptane. The heptane was evaporated under vacuum to provide a yellow solid. The yellow solid was dissolved in 300 mL of THF and 25 mL of 10% aqueous sodium hydroxide was added. After stirring for about 3 hours, the mixture was adjusted to pH 7 by addition of 4N HCl (about 16 mL). The THF was evaporated under vacuum to leave an aqueous residue, to which was added 300 mL of distilled water. After stirring this mixture, a fine suspension of solids resulted. The solid was collected by filtration and the filtered solid was washed with water (1400 mL) in several portions, resulting in the desired product.

1. customThe organic layer was then separated
2. temperatureheated at about 60° C. for 12 hours
3. stirringstirred at 20–25° C. for 6 hours
4. addition3 mL of ammonium hydroxide (29% ammonia in water) was added
5. stirringthe mixture stirred for 1.5 hours
6. washThe resulting mixture was washed with 4×200 mL of 10% aqueous potassium carbonate
7. customthe organic layer was separated
8. customevaporated under vacuum
9. customto provide an oil
10. customThe heptane was evaporated under vacuum
11. customto provide a yellow solid
12. stirringAfter stirring for about 3 hours
13. customThe THF was evaporated under vacuum
14. customto leave an aqueous residue
15. stirringAfter stirring this mixture
16. additiona fine suspension of solids
17. customresulted
18. filtrationThe solid was collected by filtration
19. washthe filtered solid was washed with water (1400 mL) in several portions