Methanol → Tiglic acid
反応式
[CH3:1]/[C:2](=[CH:5]\[CH3:6])/C#N.[CH3:7]/[C:8](=[CH:11]/[CH3:12])/C#N.C(NC(=[O:18])C)C.C(#N)C.[CH3:22][OH:23]>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH3:1]/[C:2](=[CH:5]\[CH3:6])/[C:22]([OH:18])=[O:23].[CH3:7]/[C:8](=[CH:11]/[CH3:12])/[C:22]([OH:18])=[O:23]参加物
反応条件
- 温度
- 25 °C
実験手順
Reaction of a 1:1 Mixture of (E)- and (Z)2-Methyl-2-Butenenitrile with E. coli SS1001 Cells A 5.0 mL suspension of 0.500 g (wet cell paste) E. coli SS1001 cells (ATCC PTA-1177) in 50 mM potassium phosphate buffer (pH 7.0) was added to a mixture containing 4.91 mL of 50 mM potassium phosphate buffer (pH 7.0), 38.4 mg of (E)-2-methyl-2-butenenitrile (47.4 mM final concentration of (E)-2-methyl-2-butenenitrile), and 37.7 mg of (Z)-2-methyl-2-butenenitrile (46.5 mM final concentration of (Z)-2-methyl-2-butenenitrile), and the resulting suspension stirred at 25° C. Samples (0.100 mL) were mixed with 0.900 mL of 60 mM N-ethylacetamide (HPLC external standard) in 1:1 acetonitrile:methanol, the resulting mixture was mixed, centrifuged, and the supernatant analyzed by HPLC for (E)- and (Z)-2-methyl-2-butenenitrile and (E)- and (Z)-2-methyl-2-butenoic acid. After 23 h, the conversions of (E)-2-methyl-2-butenenitrile and (Z)-2-methyl-2-butenenitrile were 100% and 0%, respectively, and the yields of (E)-2-methyl-2-butenoic acid and (Z)-2-methyl-2-butenoic acid were 100% and 0%, respectively.
出典と根拠
- ORDレコード
- ord-9410b4251a3140bb909c695ec5863478
- 出典データセット
- uspto-grants-2007_10
- 特許番号
- US07285410B2
反応ノート
読み込み中…