Creatine → Camp

HRID 1441207

反応式

HRID 1441207 の反応式
[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[C:29]3[N:28]=[CH:27][N:26]=[C:24]([NH2:25])[C:23]=3[N:22]=[CH:21]2)[C@H:17]([OH:30])[C@@H:16]1O)([O:4]P(OP(O)(O)=O)(O)=O)(=[O:3])[OH:2].P(OC[C@H]1O[C@@H](N2C3N=C(N)NC(=O)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.P(O)(O)(O)=O.O=C(CN(C(=N)N)C)O.O=C(CN(C(=N)N)C)O.CC(CN1C(=O)N(C)C(=O)C2NC=NC1=2)C>C(O)C(N)(CO)CO>[CH:27]1[N:26]=[C:24]([NH2:25])[C:23]2[N:22]=[CH:21][N:20]([C@@H:18]3[O:19][C@@H:15]4[CH2:14][O:13][P:1]([OH:2])([O:3][C@H:16]4[C@H:17]3[OH:30])=[O:4])[C:29]=2[N:28]=1

参加物

7

反応物 5

HCID 586CreatineHCID 37583-Isobutyl-1-methylxanthineHCID 59575'-AtpHCID 186298792-[Carbamimidoyl(methyl)amino]acetic acid;phosphoric acidHCID 135398633Guanosine Triphosphate

生成物 1

HCID 6076Camp

試薬・触媒・溶媒 1

HCID 6503Tris(hydroxymethyl)aminomethane溶媒

実験手順

Membranes are incubated in 25 mM Tris (pH 7.6), 0.2% BSA, 2.6 mM Mg, 0.8 mM ATP, 0.1 mM GTP, 5 mM creatine phosphate, 50 U/ml creatine kinase, 0.2 mM IBMX at 32° C. Prospective ligands are added and incubation is continued for another 15 minutes. The amount of cAMP produced is assayed using an fluorescent immunoassay method.

後処理

  1. customat 32° C
  2. additionProspective ligands are added

出典と根拠

ORDレコード
ord-7ecdaa8541524bb59512d24ab4b045aa
出典データセット
uspto-grants-2010_02
特許番号
US07655396B1

関連ノート

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