反応式
[O:1]=[CH:2][C@H:3]([C@@H:5]([C@@H:7]([C@H:9]([CH3:11])O)[OH:8])[OH:6])[OH:4].C=C(OP(O)(O)=O)[C:14](O)=[O:15].P([O:34][CH2:35][C@H:36]1[O:40][C@@H:39]([N:41]2[C:50]3N=CN=C(N)[C:44]=3N=C2)[C@H:38]([OH:51])[C@@H:37]1[OH:52])(OP(OP(O)(O)=O)(O)=O)(=O)O.P(OC[C@H]1O[C@@H](N2C3N=C(N)NC(=O)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)[OH:54].[Mg+2].[Cl-].[Cl-].C(O)C(N)(CO)CO.Cl.[CH3:97][CH:98]1[O:103][CH:102]([O:104]P(OP(OCC2OC(N3C4NC(N)=NC(=O)C=4N=C3)C(O)C2O)(O)=O)(O)=O)[CH:101]([OH:132])[CH:100]([OH:133])[CH:99]1[OH:134].C([O-])(=O)C(C)=O>>[CH3:97][C@@H:98]1[O:103][C@@H:102]([O:104][C@H:9]2[C@H:11]([O:51][C@H:38]3[C@H:37]([OH:52])[C@@H:36]([CH2:35][OH:34])[O:40][C@@H:14]([OH:15])[C@@H:39]3[NH:41][C:50]([CH3:44])=[O:54])[O:4][C@H:3]([CH2:2][OH:1])[C@H:5]([OH:6])[C@@H:7]2[OH:8])[C@@H:101]([OH:132])[C@H:100]([OH:133])[C@@H:99]1[OH:134]参加物
9
反応物 8
生成物 1
実験手順
5 mmol Gb5 with linker, 5 mmol fucose, 12 mmol Phosphoenolpyruvic acid (PEP), 0.25 mmol ATP, 0.25 mmol GTP with 10 mM MgCl2 were added into 100 mM Tris-HCl buffer (pH 7.5). The reaction was initiated by addition suitable amount of α-1,2-fucosyltransferase, L-fucokinase/GDP-fucose pyrophosphorylase (FKP), pyruvate kinase (PK) and pyrophosphatase (PPA). The flask was placed into an incubator at 16˜50° C. with gentle shaking. The reaction was monitored by TLC. More enzymes are added if the reaction stops. The reaction is stopped when no more starting material is observed by TLC. The Globo H product was purified by 18C reverse phase column in 94% yield.
後処理
- customwas placed into an incubator at 16˜50° C. with gentle shaking
- additionMore enzymes are added if the reaction
- customThe Globo H product was purified by 18C reverse phase column in 94% yield
出典と根拠
- ORDレコード
- ord-b708590dea1244efa2e9bf3b0eedc99c
- 出典データセット
- uspto-grants-2016_05
- 特許番号
- US09340812B2
関連ノート
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