[Cl-].[Na+].[Cl-].[K+].O=[CH:6][C@@H:7]([C@H:9]([C@@H:11]([C@@H:13]([CH2:15]O)O)O)O)O.[Cl-].[Ca+2].[Cl-].P([O-])(O)(O)=O.[K+].S([O-])([O-])(=O)=O.[Mg+2].[NH:32]([C:37]([CH2:42]O)(CO)CO)[CH2:33]C(O)=O.C1[N:49](CCCS(O)(=O)=O)[CH2:48]COC1.[OH-].[Na+]>>[N:49]1[CH:48]=[C:11]([CH:13]2[CH2:15][CH2:42][CH2:37][N:32]2[CH3:33])[CH:9]=[CH:7][CH:6]=1The activity at pH 8.0 was determined using a buffer comprising 125 mM sodium chloride, 4.8 mM potassium chloride, 5.6 mM D-glucose, 1.2 mM calcium chloride, 1.2 mM potassium dihydrogen phosphate, 1.2 mM magnesium sulfate, and 25 mM tricine. The activity at pH 7.0 was further determined using a buffer comprising 125 mM sodium chloride, 4.8 mM potassium chloride, 5.6 mM D-glucose, 1.2 mM calcium chloride, 1.2 mM potassium dihydrogen phosphate, 1.2 mM magnesium sulfate, and 25 mM MOPS-NaOH.
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