O=C[C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)O)O)[OH:4].S([O-])([O-])(=O)=O.[NH4+:18].[NH4+:19].P([O-])(O)(O)=O.[K+].P([O-])([O-])(O)=O.[K+].[K+].NC(N)=O.[OH2:37].O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].Cl.S1C=CC=CC1.OC(CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=O.C(=O)([O-])[O-].[Ca+2].S(=O)(=O)(O)O>O.O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Fe+2].O.O.O.O.O.S([O-])([O-])(=O)=O.[Mn+2]>[NH2:18][C@H:5]([C:3]([OH:4])=[O:37])[CH2:7][CH2:9][C:11](=[O:12])[NH2:19]
3 ml of each of the seed culture broths was inoculated to a 250-ml baffled conical flask containing 30 ml of a main culture medium [a medium comprising 50 g of glucose, 20 g of ammonium sulfate, 0.5 g of potassium dihydrogen phosphate, 0.5 g of dipotassium hydrogen phosphate, 2 g of urea, 0.5 g of magnesium sulfate heptahydrate, 2 mg of iron sulfate heptahydrate, 2.5 mg of manganese sulfate pentahydrate, 1 mg of thiamin hydrochloride, 100 μg of biotin, and 30 g of calcium carbonate, contained in 1 L of water, sterilized and then adjusted to pH 7.0 with sulfuric acid], and cultured under the conditions of 30° C. and 220 rpm for 16 hours.
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