반응식
O=[C:2]([O-:13])[C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[Cl-:14].[K+].[Mg+2].[Cl-].[Cl-].C1N(CCO)CCN(CCS(O)(=O)=O)C1.C(N(CC(O)=O)CC(O)=O)COCCOCCN(CC(O)=O)CC(O)=O.[Cl-].[Cl-].[Ca+2].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[OH-].[K+].[Cl-].[Cs+].CC[N+](C[C:87]([NH:89][C:90]1[C:95]([CH3:96])=[CH:94][CH:93]=[CH:92][C:91]=1C)=O)(CC)CC.[Br-:98].[Na+].[Cl-]>>[CH:91]1[C:90]2[NH:89][CH:87]=[C:96]([O:12][C@@H:11]3[O:4][C@H:3]([CH2:2][OH:13])[C@H:5]([OH:6])[C@H:7]([OH:8])[C@H:9]3[OH:10])[C:95]=2[C:94]([Cl:14])=[C:93]([Br:98])[CH:92]=1참가 물질
반응물 11
HCID 4873Potassium Chloride2회
HCID 5234Sodium Chloride
HCID 6207Egta2회
HCID 14797Potassium Hydroxide
HCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid2회
HCID 24293Cesium Chloride
HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
HCID 5284359Calcium Chloride3회
HCID 5360315Magnesium Chloride
HCID 6419706Gluconate
HCID 9884487QX-314 bromide3회생성물 1
실험 절차
Electrophysiology. Cells were analyzed at indicated time points after dox induction. Action potentials were recorded with current-clamp whole-cell configuration. The pipette solution for current-clamp experiments contained (in mM) 123 K-gluconate, 10 KCl, 1 MgCl2, 10 HEPES, 1 EGTA, 0.1 CaCl2, 1 K2ATP, 0.2 Na4GTP and 4 glucose, pH adjusted to 7.2 with KOH. Membrane potentials were kept around −65 to −70 mV, and step currents were injected to elicit action potentials. For voltage-clamp experiments, the internal solution contained (in mM) CsCl 135, HEPES 10, EGTA 1, Mg-ATP 4, Na4GTP 0.4, and QX-314 10, pH7.4. The bath solution contained (in mM): NaCl 140, KCl 5, CaCl2 2, MgCl2 2, HEPES 10, and glucose 10, pH7.4. Whole-cell currents including Na+ and K+ currents were recorded at a holding potential of −70 mV, voltage steps ranging from −80 mV to +90 mV were delivered at 10 mV increments.
출처 및 근거
- ORD 기록
- ord-8c17c882d8d64bd6bd87b6e5079851fa
- 출처 데이터셋
- uspto-grants-2015_06
- 특허 번호
- US09057053B2
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