Nicotinamide → 3-Amino-5-hydroxybenzoic acid

HRID 418976

반응식

HRID 418976 반응식
[O:1]=[CH:2][C@@H:3]([C@H]([C@@H]([C@@H](CO)O)O)O)O.O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].S([O-])([O-])(=O)=O.[NH4+].[NH4+].P([O-])(O)(O)=O.[K+].[CH3:39][C:40]1[N+:44](CC2C=NC(C)=NC=2N)=CS[C:41]=1[CH2:54][CH2:55][OH:56].Cl.[Cl-].[OH:59]C(CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=O.C(N)(=O)C1C=CC=NC=1>O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Fe+2].O.O.O.O.O.S([O-])([O-])(=O)=O.[Mn+2]>[CH:41]1[C:40]([NH2:39])=[CH:44][C:2]([OH:1])=[CH:3][C:54]=1[C:55]([OH:56])=[O:59]

참가 물질

10

반응물 7

HCID 936NicotinamideHCID 6202Thiamine HydrochlorideHCID 24843Magnesium Sulfate HeptahydrateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium Sulfate

생성물 1

HCID 1271153-Amino-5-hydroxybenzoic acid

시약·촉매·용매 2

HCID 62662Ferrous sulfate heptahydrate촉매HCID 22138077Manganese II sulfate pentahydrate촉매

실험 절차

The C. glutamicum AJ110135 having an enhanced AKfbr gene and the C. glutamicum AJ110135 having an enhanced PC gene selected in Example 3 (2) and (3), respectively were cultured in the flask evaluation medium (100 g of glucose, 1 g of magnesium sulfate heptahydrate, 55 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 0.01 g of iron sulfate heptahydrate, 0.01 g of manganese sulfate pentahydrate, 2 mg of thiamine hydrochloride, 0.5 mg of biotin, 5 mg of nicotine amide, 1.05 g of soy concentrate (hydrolyzed soy protein, as total nitrogen content) and 50 g of calcium carbonate were adjusted in 1 L of water to pH 7.2, and kanamycin was added at a final concentration of 25 mg/L and chloramphenicol was added at a final concentration of 5.0 mg/L) at 30° C. for 71 hours at 120 rpm. As the control experiment, the C. glutamicum AJ110135 with the pVC7 was cultured in the above flask evaluation medium with kanamycin at a final concentration of 25 mg/L for 71 hours. As a result, glucose was completely consumed in all of the experiments. 1.0 g/L of 3,4-AHBA was accumulated in the culture of C. glutamicum AJ110135 having the enhanced AKfbr gene, and 0.6 g/L of 3,4-AHBA was accumulated in the culture of C. glutamicum AJ110135 having the enhanced PC gene (Table 2). Meanwhile, 0.5 g/L of 3,4-AHBA was accumulated in the culture of C. glutamicum AJ110135 having introduced pVC7 as the control experiment. From the above results, the ability to form AHBA was improved in C. glutamicum AJ110135 having the enhanced AKfbr gene and C. glutamicum AJ110135 having the enhanced PC gene compared with C. glutamicum AJ110135 as the control.

후처리

  1. concentrationconcentrate (hydrolyzed soy protein, as total nitrogen content) and 50 g of calcium carbonate
  2. additionwas added at a final concentration of 25 mg/L and chloramphenicol
  3. additionwas added at a final concentration of 5.0 mg/L) at 30° C. for 71 hours at 120 rpm
  4. customAs a result, glucose was completely consumed in all of the experiments
  5. additionglutamicum AJ110135 having introduced pVC7 as the control experiment

출처 및 근거

ORD 기록
ord-c2cff203c9cd412ba440733db348b2bd
출처 데이터셋
uspto-grants-2012_01
특허 번호
US08093346B2

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