Thiamine Hydrochloride → HCID 178419078, 화합물

HRID 487047

반응식

HRID 487047 반응식
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].[K].S([O-])([O-])(=O)=O.[Mg+2].CC1[N+:32]([CH2:33][C:34]2[CH:35]=[N:36][C:37](C)=[N:38][C:39]=2N)=CSC=1CCO.Cl.[Cl-].[OH:47][C:48](CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:49].C([O-])(=O)C.[NH4+]>S([O-])([O-])(=O)=O.[Mn+2]>[NH2:32][C@H:33]([C:48]([OH:49])=[O:47])[CH2:34][C:35]1[N:36]=[CH:37][NH:38][CH:39]=1

참가 물질

9

반응물 7

HCID 6202Thiamine HydrochlorideHCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 517165Ammonium AcetateHCID 5462222PotassiumHCID 6097028Ammonium Sulfate

생성물 1

HCID 178419078HCID 178419078, 화합물

시약·촉매·용매 1

HCID 24580Manganese Sulfate촉매

실험 절차

An aqueous solution medium having a composition of 10% of glucose, 0.5% of ammonium sulfate, 0.15% of potassium primary phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate, 0.001% of manganese sulfate, 300 μg/l of thiamine hydrochloride, 350 μg/l of biotin, 5 ml/dl of Aji-Eki® and 0.5% of ammonium acetate, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in an autoclave, the various L-histidine-producing bacterial strains shown in Table 14, which had been previously grown in bouillon slants at 30° C. for 24 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 6.5 by the addition of ammonia gas. After completion of the fermentation, the yield of L-histidine produced and accumulated in the solution based on glucose was determined and is shown in Table 14.

후처리

  1. additionat pH 7.0, was charged in an amount of 300 ml
  2. custominto separate small sized glass jar fermenters

출처 및 근거

ORD 기록
ord-ae9a1e9a8cdf48b497f84afe1eac13bf
출처 데이터셋
uspto-grants-1994_03
특허 번호
US05294547

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