反应详情

Protected arginine, i.e., tert-butyloxycarbonylnitro-arginine is dissolved in a suitable solvent such as dimethylformamide and tetrahydrofuran. The dissolved protected amino acid is then cooled to a temperature slightly below the freezing point (0° C), whereupon isobutyl chlorocarbonate and triethylamine is added with stirring. After a short period, glycinamide dissolved in dimethylformamide is added. The mixture is then permitted to warm to room temperature and stirred for a period of several hours and then filtered. The filtrate is recovered and evaporated to dryness and then extracted with dimethylformamide. The dimethylformamide solution is precipitated with ethylacetate to yield the dipeptide, i.e., protected arginyl-glycinamide.

1. additionis added
2. waitAfter a short period
3. additionis added
4. stirringstirred for a period of several hours
5. filtrationfiltered
6. customThe filtrate is recovered
7. customevaporated to dryness
8. extractionextracted with dimethylformamide
9. customThe dimethylformamide solution is precipitated with ethylacetate