1,2-Dichloroethane → HCID 16225597, compound

To a cooled (−78° C.) solution of the title compound of Example 15 Step B (65.0 mg, 0.17 mmol) in THF (1 mL) was added KHMDS (0.660 mL, 0.5 M in toluene, 0.33 mmol). After 30 min, methyl 4-bromomethylbenzoate (76.0 mg, 0.33 mmol) was added, and the resultant mixture was allowed to warm slowly to room temperature over 15 h. The mixture was then quenched by addition of sat. aq. NaHCO3, and the aqueous phase was extracted with EtOAc. The organic phase was dried over anhydrous Na2SO4, and concentrated in vacuo. LCMS A, tr=4.40 min, m/z 542.4 [M+H]+. To a solution of the crude adduct obtained above in 1,4-dioxane (2 mL) was added a solution of LiOH (1 mL, 2.0 N in H2O, 2.0 mmol), and the resultant mixture was stirred at 50° C. for 2 h. The mixture was allowed to cool to room temperature whereupon it was quenched by the addition of 2 N aq. HCl. The aqueous phase was extracted with EtOAc, and the organic phase was dried over Na2SO4 and concentrated in vacuo. To the crude carboxylic acid obtained above were added EDC (96.0 mg, 0.50 mmol), HOBt (68.0 mg, 0.50 mmol), and β-alanine tert-butyl ester hydrochloride (91.0 mg, 0.50 mmol). The resultant mixture was dissolved in DMF (1 mL), DIEA (0.177 mL, 1.0 mmol) was added, and the reaction mixture was stirred at 50° C. for 4 h. The reaction mixture was quenched by addition of sat. aq. NH4Cl, and the aqueous phase was extracted with EtOAc. The organic phase was concentrated in vacuo. Purification by flash chromatography on silica gel (0 to 50%, then 50 to 100% EtOAc in hexanes) provided the amide product: LCMS A, tr=4.31 min, m/z 599.4 [M−tBu+H]+. The amide obtained above was dissolved in CH2Cl2 (1 mL) and TFA (1 mL). After 30 min, the mixture was concentrated in vacuo. Purification by reverse phase HPLC (75 to 100% CH3CN in H2O, each with 0.1% v/v TFA) provided the title compound: LCMS A, tr=3.90 min, m/z 599.4 [M+H]+; 1H NMR (500 MHz, d6DMSO) δ 8.35 (t, J=5.5 Hz, 1H), 7.85 (d, J=8.5 Hz, 1H), 7.81 (d, J=2.0 Hz, 1H), 7.74 (t, J=8.0 Hz, 1H), 7.66 (s, 1H), 7.63 (d, J=8.0 Hz, 2H), 7.28 (dd, J=9.0, 2.0 Hz, 1H), 7.21 (d, J=8.0 Hz, 2H), 6.92 (d, J=9.0 Hz, 1H), 3.81-3.77 (m, 1H), 2.94-2.83 (m, 2H), 2.42 (t, J=7.0 Hz, 2H), 1.02 (s, 9H), α-NH β-alanine methylene group obscured by residual H2O signal.

1. customThe mixture was then quenched by addition of sat. aq. NaHCO3
2. extractionthe aqueous phase was extracted with EtOAc
3. dry with materialThe organic phase was dried over anhydrous Na2SO4
4. concentrationconcentrated in vacuo
5. customtr=4.40 min, m/z 542.4 [M+H]+
6. customTo a solution of the crude adduct obtained above in 1,4-dioxane (2 mL)
7. temperatureto cool to room temperature whereupon it
8. customwas quenched by the addition of 2 N aq. HCl
9. extractionThe aqueous phase was extracted with EtOAc
10. dry with materialthe organic phase was dried over Na2SO4
11. concentrationconcentrated in vacuo
12. customTo the crude carboxylic acid obtained
13. customThe reaction mixture was quenched by addition of sat. aq. NH4Cl
14. extractionthe aqueous phase was extracted with EtOAc
15. concentrationThe organic phase was concentrated in vacuo
16. customPurification by flash chromatography on silica gel (0 to 50%

ORD record

ord-e0db42bf9f27478cabbb7e26fe412525

Source dataset

uspto-grants-2009_10

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07598398B2