Potassium Chloride → HCID 178508912, compound

HRID 1954186Notes

Reaction equation

HRID 1954186 reaction equation
[CH:1]([N:3]([CH2:22][CH:23]1[NH:34][C:33]2[C:31](=[O:32])[NH:30][C:28]([NH2:29])=[N:27][C:26]=2[NH:25][CH2:24]1)[C:4]1[CH:21]=[CH:20][C:7]([C:8](=[O:19])[NH:9][C@H:10]([C:16]([OH:18])=[O:17])[CH2:11][CH2:12][C:13]([O-:15])=[O:14])=[CH:6][CH:5]=1)=[O:2].C([O-])=O.C1COCC1.P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.N(CCO)(CCO)CCO.C([O-])=O.[NH4+].[Mg+2].[Cl-].[Cl-].[Cl-].[K+].P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>O.C1COCC1>[CH2:24]1[NH:25][C:26]2[NH:27][C:28]([NH2:29])=[N:30][C:31](=[O:32])[C:33]=2[NH:34][CH:23]1[CH2:22][N:3]([C:4]1[CH:5]=[CH:6][C:7]([C:8]([NH:9][C@H:10]([C:16]([OH:18])=[O:17])[CH2:11][CH2:12][C:13]([OH:15])=[O:14])=[O:19])=[CH:20][CH:21]=1)[CH:1]=[O:2]

Participants

10

Reactant 7

HCID 4873Potassium ChlorideHCID 59575'-AtpHCID 7618TriethanolamineHCID 2723923Ammonium formateHCID 5360315Magnesium ChlorideHCID 131722006HCID 131722006, compoundHCID 137182957HCID 137182957, compound

Product 1

HCID 178508912HCID 178508912, compound

Reagents, catalysts & solvents 2

HCID 962WaterSolventHCID 8028TetrahydrofuranSolvent

Procedure

10-formyltetrahydrofolate-synthetase catalyzes the reaction, formate+THF+ATP→10-formyl-THF+ADP+Pi. A test tube containing 100 μL 1 M triethanolamine buffer (pH 7.5), 100 μL 1 M ammonium formate (pH 7.5), 100 μL 25 mM MgCl2, 100 μL 2 M KCl, 100 μL 10 mM THF, freshly made 100 μL 20 mM ATP, and 390 μL water was pre-incubated for 2 min at 30° C. A 10 μL aliquot of the extracted sample containing enzyme was added and incubated 15 min at 30° C. The reaction was terminated by adding 2 mL 0.36 N HCl. The 10-formyl-THF formed during the incubation period was converted to 5,10-methenyl-THF by the acid. The absorbency was measured at 350 nm against a water blank and the 5,10-methenyl-THF formed was determined by using an extinction coefficient of 24,900 M-1 cm-1.

Workup

  1. additionA 10 μL aliquot of the extracted sample containing enzyme
  2. additionwas added
  3. waitincubated 15 min at 30° C
  4. customThe reaction was terminated
  5. additionby adding 2 mL 0.36 N HCl

Sources & evidence

ORD record
ord-a6c17b6d46424c62a42bf7ac1568f471
Source dataset
uspto-grants-2000_09
Patent number
US06121195

Reaction notes

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