Carbon Dioxide → HCID 11634971, compound

2-{(S)-2-[2-tert-Butoxycarbonylamino-2-(1-methyl-1 H-benzoimidazol-5-yl)-acetylamino]-3-phenyl-propionylamino)-thiazole-4-carboxylic acid methyl ester (185 mg, 0.193 mmol) was dissolved at 0° C. in a 30% v/v solution of trifluoroacetic acid in dichloromethane (10 mL, 38.9 mmol). The reaction mixture was slowly warmed to room temperature and stirred for approximately 3.5 hours. The reaction mixture was then cooled to 0° C. and neutralized by the addition of solid sodium bicarbonate until there was no further evolution of carbon dioxide. The reaction mixture was then partitioned between dichloromethane and water and the water layer thoroughly extracted with dichloromethane until no products could be detected in the organic extract as judged by absorption of ultraviolet radiation on silica gel thin layer chromatography plates containing a fluorescent indicator which absorbed at 254 nm. The combined organic layers were dried over sodium sulfate, filtered and concentrated. The residue was dissolved in tetrahydrofuran (≈5 mL), diisopropylethyl amine (180 μL, 1.00 mmol) added and the resulting solution was transferred via cannula to a solution of diphosgene (20 μL, 0.18 mmol) in tetrahydrofuran (≈10 mL) at 0° C. After stirring for 10 minutes the reaction mixture was partitioned between ethyl acetate and water, the water layer separated and extracted with ethyl acetate until no products could be detected in the organic extract as judged by absorption of ultraviolet radiation on silica gel thin layer Chromatography plates containing a fluorescent indicator which absorbed at 254 nm. The combined organic extracts were dried over sodium sulfate, filtered and concentrated. The residue was purified by chromatography over silica gel gradient eluted between 0 and 5% v/v methanol in dichloromethane and the isolated product precipitated from a dichloromethane solution with an exess of hexanes to afford 2-{(S)-2-[4-(1-methyl-1H-benzoimidazol-5-yl)-2,5-dioxo-imidazolidin-1-yl]-3-phenyl-propionylamino}-thiazole-4-carboxylic acid methyl ester as an off-white solid (60 mg, 60%).

1. temperatureThe reaction mixture was then cooled to 0° C.
2. customThe reaction mixture was then partitioned between dichloromethane and water
3. extractionthe water layer thoroughly extracted with dichloromethane until no products
4. extractionorganic extract
5. customas judged by absorption of ultraviolet radiation on silica gel thin layer chromatography plates
6. additioncontaining a fluorescent indicator which
7. customabsorbed at 254 nm
8. dry with materialThe combined organic layers were dried over sodium sulfate
9. filtrationfiltered
10. concentrationconcentrated
11. dissolutionThe residue was dissolved in tetrahydrofuran (≈5 mL)
12. stirringAfter stirring for 10 minutes the reaction mixture
13. customwas partitioned between ethyl acetate and water
14. customthe water layer separated
15. extractionextracted with ethyl acetate until no products
16. extractionorganic extract
17. customas judged by absorption of ultraviolet radiation on silica gel thin layer Chromatography plates
18. additioncontaining a fluorescent indicator which
19. customabsorbed at 254 nm
20. dry with materialThe combined organic extracts were dried over sodium sulfate
21. filtrationfiltered
22. concentrationconcentrated
23. customThe residue was purified by chromatography over silica gel gradient
24. washeluted between 0 and 5% v/v methanol in dichloromethane
25. customthe isolated product precipitated from a dichloromethane solution with an exess of hexanes

ORD record

ord-4a30a8a2224c4ef9b2d3af30638a7873

Source dataset

uspto-grants-2008_05

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07371869B2