Hydrochloric Acid → Val-Ala-Asp(OtBu)OH

Temperature

-15 °C

Condensation with Asp(OtBu)OH: Asp(OtBu)OH (2.51 g) was dissolved in 40 mL of dry DMF under argon and 8.2 mL of bis(trimethylsilyl)acetamide (BSA) and the reaction was allowed to stir 40 minutes. In a separate flask, Mu-Val-Ala-OH (4.0 g) was dissolved in 200 mL of dry THF under argon and the resulting solution was cooled to -15° C. and one equivalent of N-methylmorpholine was added followed by one equivalent of isobutylchloroformate and the resulting mixture was allowed to stir 20 minutes and then the first reaction was then poured into the second reaction and both were maintained at -15° C. for one hour and then allowed to come slowly to room temperature and to stir overnight. The reaction was poured into 150 mL of 1N HCl and extracted with 2×200 mL of ethyl acetate. The combined organic fractions were washed with 15 mL 1N HCl, brine (50 mL), dried over MgSO4 (with decolorizing carbon), filtered, and the solvents were removed under reduced pressure to yield 4.89 g of Mu-Val-Ala-Asp(OtBu)OH.

1. stirringto stir 20 minutes
2. customthe first reaction
3. additionwas then poured into the second reaction
4. temperatureboth were maintained at -15° C. for one hour
5. customto come slowly to room temperature
6. stirringto stir overnight
7. extractionextracted with 2×200 mL of ethyl acetate
8. washThe combined organic fractions were washed with 15 mL 1N HCl, brine (50 mL)
9. dry with materialdried over MgSO4 (with decolorizing carbon)
10. filtrationfiltered
11. customthe solvents were removed under reduced pressure

ORD record

ord-ab053d66bdd34e569e5f37e92683cddd

Source dataset

uspto-grants-1998_02

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05714484