4-Methylmorpholine → HCID 135850842, compound

Temperature

0 °C

Under an argon atmosphere 1H-1,2,3-benzotriazol-1-yl{[(benzyloxy)carbonyl]amino}acetic acid (6.71 g, 20.6 mmol) was suspended in anhydrous methylene chloride (92 ml) and cooled to 0° C. in an ice bath. Oxalyl chloride (2.61 g, 20.6 mmol), then N,N-dimethylformamide (38 ml) were added dropwise to the suspension. The reaction mixture was stirred at 0° C. in an ice bath for 30 minutes, at which point no further gas evolution was noted. Then a solution of 1-(2-amino-4-bromophenyl)ethanone (4.0 g, 18.7 mmol) and 4-methylmorpholine (2.84 g, 28.0 mmol) in anhydrous methylene chloride (60 ml) was added dropwise. The reaction mixture was allowed to warm to room temperature over 12 hours, then quenched with water (200 ml), and extracted with ethyl acetate (3×250 ml). The organic layers were combined, dried over magnesium sulfate, filtered and concentrated to give a semi-solid which was dissolved in tetrahydrofuran (120 ml) and methyl alcohol (35 ml). Ammonia gas was bubbled through this solution for 2.5 hours. The reaction was then concentrated to a viscous light brown oil. The oil was dissolved in acetic acid (120 ml) and ammonium acetate (4.3 g, 56.1 mmol) was added in one portion and stirred for 12 hours. The reaction mixture was diluted with water (100 ml) and then made basic (pH=10) with 25% sodium hydroxide, while stirring in an ice bath. The aqueous solution was then extracted with ethyl acetate (3×500 ml) and the organic layers combined, dried over magnesium sulfate, filtered and concentrated. The resulting residue was purified on silica gel, eluting with 40% ethyl acetate in hexanes to give the title compound (4 g, 53%). 1H NMR (CDCl3, 300 MHz): δ 9.90 (s-br, 1 H), 7.42-7.31 (m, 6 H), 7.12 (d, 1 H, 1.5 Hz), 7.06-7.03 (m, 1 H), 5.18-5.08 (m, 3 H), 2.50 (s, 3 H) ppm.

1. temperatureto warm to room temperature over 12 hours
2. customquenched with water (200 ml)
3. extractionextracted with ethyl acetate (3×250 ml)
4. dry with materialdried over magnesium sulfate
5. filtrationfiltered
6. concentrationconcentrated
7. customto give a semi-solid which
8. customAmmonia gas was bubbled through this solution for 2.5 hours
9. concentrationThe reaction was then concentrated to a viscous light brown oil
10. dissolutionThe oil was dissolved in acetic acid (120 ml)
11. stirringstirred for 12 hours
12. stirringwhile stirring in an ice bath
13. extractionThe aqueous solution was then extracted with ethyl acetate (3×500 ml)
14. dry with materialdried over magnesium sulfate
15. filtrationfiltered
16. concentrationconcentrated
17. customThe resulting residue was purified on silica gel
18. washeluting with 40% ethyl acetate in hexanes

ORD record

ord-a570c9ed3ecf4d0c98b0f7f6d23f208e

Source dataset

uspto-grants-2008_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07354914B2