Triethylamine → 2'-Deoxy-5-ethynyl-5'-O-[(4-methoxyphenyl)(diphenyl)methyl]uridine

2'-Deoxy-5-iodo-5'-O-(4-methoxytrityl)uridine (1.2 g, 1.8 mmol) was dissolved in 6 ml of anhydrous N,N-dimethylformamide (DMF) and the solution was vigorously deoxygenated with nitrogen for 30 min. To the magnetically stirred solution was added 142 mg (0.20 mmol) bis(triphenylphosphine)palladium(II) chloride, 76 mg (0.40 mmol) copper (I) iodide, 0.53 ml (5.40 mmol) of trimethylsilylacetylene and 0.56 ml (4.0 mmol) of triethylamine (Aldrich Chemical Company, Milwaukee, Wis. 53233). The following day identical portions of bis(triphenylphosphine)palladium(II)chloride, copper (I) iodide, trimethylsilylacetylene and triethylamine were added. The reaction was allowed to stir for an additional 5 hrs before being poured into 100 ml ethyl acetate in a separating funnel. The ethyl acetate solution was extracted with 3×30 ml aqueous 5% ethylenediamine tetra acetic acid disodium salt and washed with 1×30 ml water. Ethyl acetate was removed on the rotary evaporator to give a black foam. This foam was dissolved in 5 ml of acetonitrile and 222 mg (1.5 mmol) of tetraethyl ammonium fluoride hydrate (Aldrich Chemical Company, Milwaukee, Wis. 53233) was added and the solution was magnetically stirred for 1 hr. After this period the reaction mixture was loaded directly onto a column (2.5 C 30 cm) packed with silica gel (100 g, 230-400 mesh) in chloroform. Under medium air pressure (9-12 psi) the column was first eluted with 1 L of chloroform, then 1 L of 1% methanol in chloroform and finally with 1 L of 2.5% methanol in chloroform to elute the product. The appropriate product fractions were combined and solvent was removed on a rotary evaporator to 769 mg (1.5 mmol) of a light brown foam: Rf 0.23 (silica, 95:5 chloroform: methanol) ; IR (1% KBr) acetylenic carbon-carbon stretch 2110 cm-1 ; MS (EI, Cl,) 524 (M). 1H NMR (300 MHz, DMSO - d6) δ 11.69 (s, 1H, NH), 7.94 (s, 1H, H6), 7.41-7.21 (m, 10H, Ar H), 6.90 (d, 2H, Ar H, J=8.9 Hz), 6.11-6.07 (overlapping dd, 1H, H1'), 5.30 (d, 1H, 3' OH, J=4.6 Hz), 4.24 (m, 1H, H4'), 3.74 (s, 3H OCH3), 3.41-3.11 (m, overlapping water peak, H5' & H5"), 2.27-2.18 (m, 2H, H2" & H2').

1. customthe solution was vigorously deoxygenated with nitrogen for 30 min
2. extractionThe ethyl acetate solution was extracted with 3×30 ml aqueous 5% ethylenediamine tetra acetic acid disodium salt
3. washwashed with 1×30 ml water
4. customEthyl acetate was removed on the rotary evaporator
5. customto give a black foam
6. stirringthe solution was magnetically stirred for 1 hr
7. waitAfter this period the reaction mixture was loaded directly onto a column (2.5 C 30 cm)
8. washUnder medium air pressure (9-12 psi) the column was first eluted with 1 L of chloroform
9. wash1 L of 1% methanol in chloroform and finally with 1 L of 2.5% methanol in chloroform to elute the product

ORD record

ord-15fd33c58507444fba87babc110efa01

Source dataset

uspto-grants-1998_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05843917