Hydrochloric Acid → HCID 53923993, compound

Temperature

0 °C

To a stirred solution of (R)-4-benzyl-2-oxazolidinonyl 3-[2-[N-(tert-butoxycarbonyl)-N-(4-trifluoromethylbenzyl)aminomethyl]-4-methyoxyphenyl]-2(S)-methoxycarbonylmethyl-propionamide (38.0 g, 54 mmol) in THF (300 mL) and water (100 mL) was added dropwise at 0° C. over 30 min a solution of 30% H2O2 (18.9 mL) and LiOH.H2O (2.3 g, 55 mmol) in water (62 mL). The cloudy solution was stirred for an additional 1 h at 0° C. The resulting homogeneous solution was treated slowly with a solution of sodium sulfite (34.3 g, 272 mmol) in water (175 mL) at 0° C., then was acidified with an ice-cold solution of concentrated HCl (35 mL) in water (150 mL). The reaction was extracted with ethyl acetate (2×200 mL), and the combined organic layers were washed with brine (400 mL), dried (MgSO4) and concentrated to dryness. The resulting residue was treated with 4.0 M HCl in dioxane (400 mL) with stirring at RT (slow gas evolution was observed). After 1 h, the reaction was concentrated and reconcentrated from 1:1 CHCl3/toluene (2×), then the residue (37.65 g) was taken up in dry DMF (400 mL). To this solution with stirring under argon at 0° C. in a Dewar flask were added triethylamine (15.3 mL, 109 mmol) and NaHCO3 (22.9 g, 273 mmol), followed by diphenylphosphoryl azide (13 mL, 60 mmol). After stirring for 24 h at 0° C. the reaction was concentrated to dryness. The residue was taken up in ethyl acetate (400 mL), and washed sequentially with water (300 mL) and brine (300 mL). Drying (MgSO4), concentration, and flash chromatography on silica gel (35% ethyl acetate/hexanes) gave the title compound (16.87 g, 74%) as a clear thick oil: TLC (40% EtOAc/hexane) Rf 0.50; MS (ES) m/e 422.3 (M+H)+; 1H NMR (400 MHz, CDCl3) δ 7.52 (d, J=8.1, 2H), 7.29 (d, J=8.1 Hz, 2H), 7.02 (d, J=8.5 Hz, 1H), 7.75 (dd, 1H), 6.36 (d, J=2.7 Hz, 1H), 5.18 (d, J=16.5 Hz, 1H), 4.96 (d, J=15.4 Hz, 1H), 4.48 (d, J=15.4 Hz, 1H), 3.87 (m, 1H), 3.74 (d, J=16.5 Hz, 1H), 3.73 (s, 3H), 3.71 (s, 3H), 3.08 (dd, 1H), 3.02 (dd, 1H), 2.95 (dd, 1H), 2.48 (dd, 1H).

1. extractionThe reaction was extracted with ethyl acetate (2×200 mL)
2. washthe combined organic layers were washed with brine (400 mL)
3. dry with materialdried (MgSO4)
4. concentrationconcentrated to dryness
5. additionThe resulting residue was treated with 4.0 M HCl in dioxane (400 mL)
6. stirringwith stirring at RT (slow gas evolution was observed)
7. waitAfter 1 h
8. concentrationthe reaction was concentrated
9. stirringTo this solution with stirring under argon at 0° C. in a Dewar flask
10. stirringAfter stirring for 24 h at 0° C. the reaction
11. concentrationwas concentrated to dryness
12. washwashed sequentially with water (300 mL) and brine (300 mL)
13. customDrying
14. concentration(MgSO4), concentration, and flash chromatography on silica gel (35% ethyl acetate/hexanes)

ORD record

ord-10a9eacffa5646318f499103b4b34650

Source dataset

uspto-grants-2004_11

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06825188B2