Sodium Hydroxide → L-Lysine

HRID 1362819

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HRID 1362819 반응식
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.OP([O-])(O)=O.[K+].[OH-].[Na+].S([O-])([O-])(=O)=O.[NH4+:26].[NH4+:27].Cl.S1C=CC=CC1.[OH:35][C:36]([CH2:38][CH2:39][CH2:40][CH2:41][C@H:42]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:37]>[Pt]>[NH2:26][C@H:38]([C:36]([OH:35])=[O:37])[CH2:39][CH2:40][CH2:41][CH2:42][NH2:27]

참가 물질

8

반응물 6

HCID 14798Sodium HydroxideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium SulfateHCID 67590811Thiaminhydrochloride

생성물 1

HCID 5962L-Lysine

시약·촉매·용매 1

HCID 23939Platinum촉매

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온도
120 °C

실험 절차

A medium containing 45 g/L of glucose, 15 g/L of molasses, 2 g/L (as nitrogen) of soybean protein hydrolysate, 2 g/L of KH2PO4, 5.6 g/L of NaOH, 10 g/L of ammonium sulfate, 0.8 g/L of MgSO4.7H2O, 20 mg/L of FeSO4.7H2O, 20 mg/L of MnSO4.4H2O, 0.8 mg/L of thiamin hydrochloride and 0.2 mg/L of biotin (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C., 5 platinum loops of Brevibacterium lactofermentum ATCC 31269 (refer to U.S. Pat. No. 4,066,501) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 31.5° C. and pH 7.0 for 30 hours with sufficient aeration and stirring.

후처리

  1. temperatureAfter the fermentation tank was cooled to 31.5° C.
  2. customcultured at 31.5° C.
  3. waitpH 7.0 for 30 hours with sufficient aeration

출처 및 근거

ORD 기록
ord-29426e88174549fe882c745e35c21a93
출처 데이터셋
uspto-grants-2011_02
특허 번호
US07883878B2

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